MDL number MFCD00677366
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| Related Categories | Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Centrosome, |
| species reactivity | Xenopus, canine, chicken, hamster, human, rat, bovine, mouse |
| application(s) | immunocytochemistry: 1:5,000-1:10,000 using HeLa cells |
| indirect ELISA: suitable | |
| western blot: 1:10,000 using cultured chicken fibroblast extract | |
| clone | GTU-88, monoclonal |
| antibody form | ascites fluid |
| isotype | IgG1 |
| mol wt | antigen mol wt 48 kDa |
| contains | 15 mM sodium azide |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information |
human ... TUBG1(7283) mouse ... Tubg1(103733) rat ... Tubg1(252921) |
synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH.
Gamma-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. Gamma-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. gamma-Tubulin abundance is less than 1% of the level of either α- or β-tubulin.
Monoclonal Anti-gamma-Tubulin recognizes an epitope located in the N-terminal amino acids 38-53 of gamma-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus gamma-tubulin.
The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.
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