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T6567 Sigma

Trypsin from porcine pancreas

Proteomics Grade, BioReagent, Dimethylated

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 mg in glass bottle

Application

Trypsin from porcine pancreas is used for the following applications:
• In-gel protein digestion and MALDI-TOF mass spectrometry analysis
• Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
• Surface proteome profiling of L. plantarum
• Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
• Mass spectrometry

Price and Availability


Amplified Detection

New Internal Standards for Mass Spectrometry

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YN5075000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?
This product is soluble in 1mM HCL. The preparation information is available in the technical bulletin, under the Useful links and tools, on the product page. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/t6567bul.Par.0001.File.tmp/t6567bul.pdf
What is the storage and stability of Product T6567, Trypsin from porcine pancreas?  
This information is also available in the Technical bulletin. The link is attached below. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/t6567bul.Par.0001.File.tmp/t6567bul.pdf
What is the package size of Product T6567, Trypsin from porcine pancreas?
Each vial content 20 ug of the product.
Is Product T6567, Trypsin from porcine pancreas, TPCK treated?
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?
If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank: The link to the website is as follow: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=136429
How can we digest protein using Product T6567, Trypsin from porcine pancreas?
As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
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Protocols & Articles

Articles

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Human protein extract was digested using native and recombinant trypsins in a traditional overnight 37°C digest and a rapid 2 hr 45°C protocol. In all cases, similar numbers of proteins were identifi...
Judy Boland, Nicolas Caffarelli, Gordon Nicol, Amber Henry, Jeffrey L. Turner, and Kevin Ray
MilliporeSigma, A business of Merck KGaA, Darmstadt, Germany, St. Louis MO 63103
Keywords: Mass spectrometry, Proteomics, Sequencing

HPLC Analysis of Carboxyamidomethylated Lysozyme Tryptic Digest on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamidomethylated Lysozyme Tryptic Digest on BIOshell™ A160 Peptide C18
Keywords: Chromatography, Digestions, High performance liquid chromatography, Mass spectrometry

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Protocols

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Nα-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determina...

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Stable Isotope-labeled MS Internal Standards

What happens when you combine state-of-the-art technology with scientific prowess? Full-length protein standards with the highest purity and isotopic incorporation in the industry. Stable isotope-lab...
Keywords: Atomic absorption spectroscopy, Digestions, Mass spectrometry, Phosphorylations

Peer-Reviewed Papers
15

References

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