• USA Home
  • T6634 - Thrombin from bovine plasma

EMAIL THIS PAGE TO A FRIEND
T6634 Sigma

Thrombin from bovine plasma

lyophilized powder, 600-2,000 NIH units/mg protein (biuret)

Synonym: Factor IIa

Purchase

Description

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess global haemostasis and point of care testing.

Price and Availability


Available in ELITE Grade
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
XO8950000
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

inhibitor

Product #

Description

Add to Cart

D7910 3,4-Dichloroisocoumarin, serine protease inhibitor
D0879 Diisopropylfluorophosphate
L2023 Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial
A6191 Antipain dihydrochloride from microbial source, protease inhibitor
H7380 Hirudin from leeches, lyophilized powder, 300-1,500 ATU/mg protein (ATU = antithrombin units)
H7016 Hirudin from leeches, lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)
A9141 Antithrombin III from bovine plasma, lyophilized powder, 200-400 units/mg protein (E1%/280 = 6.5)
P7626 Phenylmethanesulfonyl fluoride, ≥98.5% (GC)

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

substrate

Product #

Description

Add to Cart

B9385 Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride
S9009 Sar-Pro-Arg p-nitroanilide dihydrochloride, thrombin substrate
B7632 N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?