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T6666 Sigma

Trizma® hydrochloride

BioXtra, pH 3.5-5.0 (0.5 M in H2O), ≥99.0% (titration)

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
Quality Level   ELITE
product line   BioXtra
assay   ≥99.0% (titration)
impurities   Insoluble matter, passes filter test
ign. residue (900°C)   ≤0.2% (as SO4)
loss   ≤0.2% loss on drying, 110°C
pH   3.5-5.0 (0.5 M in H2O)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble0.5 M, clear, colorless
anion traces   sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A0.5M/260, H2O ≤0.015
  A0.5M/280, H2O ≤0.010
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Packaging

1 kg in poly bottle

50, 250 g in poly bottle

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tris Always Available

Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

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Peer-Reviewed Papers
15

References

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