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T6791 Sigma

Trizma® base

BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
Quality Level   ELITE
description   aminopeptidase substrate
product line   BioXtra
assay   ≥99.9% (titration)
impurities   ≤0.005% Insoluble matter
ign. residue (900°C)   ≤0.01% (as SO4)
loss   ≤0.5% loss on drying, 110°C
pH   10.5-12.0 (1 M in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   H2O: soluble1 M, colorless
anion traces   chloride (Cl-): ≤0.005%
  sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A1M/260, H2O ≤0.025
  A1M/280, H2O ≤0.020
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



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Protocols & Articles

Articles

Determination of Water Content in Tris-Hydroxymethylaminomethane Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

Determination of Water Content in Tris-Hydroxymethylaminomethane Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

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Peer-Reviewed Papers

References

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Developing structure-activity relationships for the prediction of hepatotoxicity. Nigel Greene et al Chem. Res. Toxicol. 23, 1215-22, (2010)

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Electrokinetic supercharging preconcentration prior to CGE analysis of DNA: sensitivity depends on buffer viscosity and electrode configuration. Ye X, Mori S, Yamada M, et al. Electrophoresis 34(4), 583-9, (2013)

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Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. Ujala Rana et al J. Inorg. Biochem. 102, 489-99, (2008)

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A predictive ligand-based Bayesian model for human drug-induced liver injury. Sean Ekins et al Drug Metab. Dispos. 38, 2302-8, (2010)

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Intensive buffering can keep pH above 7.2 for over 4 h during apnea: an experimental porcine study. Höstman S, Engström J, Hedenstierna G, et al. Acta Anaesthesiol. Scand. 57(1), 63-70, (2013)

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Quantifying the energetic contributions of desolvation and π-electron density during translesion DNA synthesis. Edward A. Motea et al Nucleic Acids Res. 39, 1623-37, (2011)

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DCAP: A Broad-Spectrum Antibiotic That Targets the Cytoplasmic Membrane of Bacteria. Eun, Y.J., et al. J. Am. Chem. Soc. 134, 11322-11325, (2012)

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Fast and scalable purification of a therapeutic full-length antibody based on process crystallization. Smejkal B, Agrawal NJ, Helk B, et al. Biotechnol. Bioeng. 110(9), 2452-61, (2013)

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Enhanced transfection efficiency of poly(N,N-dimethylaminoethyl methacrylate)-based deposition transfection by combination with tris(hydroxymethyl)aminomethane. Iwai R, Haruki R, Nemoto Y, et al. Bioconjug. Chem. 24(2), 159-66, (2013)

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Merck 14,9772

Beil. 4,IV,1903

Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 1 (1), 347:C / FT-IR 2 (1), 542:D / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / IR-Spectra (3), 203:H / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C

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