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T6793 Sigma

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

clone 6-11B-1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   plant, pig, human, monkey, hamster, invertebrates, chicken, bovine, rat, frog, protista, mouse
application(s)   dot blot: suitable
  electron microscopy: suitable
  immunocytochemistry: suitable
  indirect ELISA: suitable
  radioimmunoassay: suitable
  western blot: 1:2,000 using a bovine or rat brain preparation
clone   6-11B-1, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.

Application

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse has been used in following studies:
• immunofluorescence.
• western blotting.
• immunocytochemistry.
• immunohistochemistry.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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