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T6819 Sigma

Anti-phospho-Tau (pSer199/202) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   rat, mouse, human
application(s)   microarray: suitable
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

chemically synthesized phosphopeptide derived from the region of human tau that contains serine199 and serine202.

General description

Tau proteins are known as microtubule-associated phosphoprotein (MAP) and express mainly in neurons of central nervous system. It has a crucial role in tubulin polymerization and facilitates microtubules assembly and stability. The biological activity of tau proteins depends on degree of its phosphorylation. Anti-phospho-Tau (pSer199/202) antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Rabbit anti- phospho-Tau (pSer199/202) antibody reacts specifically with human Tau (pSer199/202) (45-68 kD).

Physical form

Solution in 100 μl Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, containing 1.0 mg/ml BSA (IgG, protease free) and 0.05% sodium azide. The amount of antibody is sufficient for 10 immunoblots.

Application

Anti-phospho-Tau (pSer199/202) antibody (diluted 1:1000 in PBS containing 0.3% Triton X-100 and 0.5% BSA) can be used in immunocytochemistry for identification of protein aggregates. It can also be used as primary antibody (diluted 1: 3000) in immunohistochemistry.

Price and Availability


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