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T6951 Sigma

TBBz

≥98% (HPLC), powder

Synonym: 4,5,6,7-Tetrabromobenzimidazole

  • CAS Number 577779-57-8

  • Empirical Formula (Hill Notation) C7H2N2Br4

  • Molecular Weight 433.72

  •  MDL number MFCD04116202

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Properties

Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology,
InChI Key   LOEIRDBRYBHAJB-UHFFFAOYSA-N
assay   ≥98% (HPLC)
form   powder
solubility   DMSO: >10 mg/mL at 60 °C, clear
storage temp.   2-8°C

Description

Preparation Note

TBBz is soluble in DMSO (60 deg C) at a concentration that is greater than 10 mg/ml.

Application

TBBz has been used as a CK2 inhibitor in HeLa cells and rat septal neurons.

Biochem/physiol Actions

TBBz is a cell-permeable Casein Kinase-2 (CK2) inhibitor. CK2 inhibitors, 4,5,6,7-tetrabromobenzotriazole (TBBt, Sigma Cat. # T0826) and rabromobenzimidazole (TBBz), the latter of which was shown to discriminate between different molecular forms of CK2 in yeast. TBBt, with a pK(a) ~5, exists in solution at physiological pH almost exclusively (>99%) as the monoanion; whereas TBBz, with a pKa ~9, is predominantly (>95%) in the neutral form, both of obvious relevance to their modes of binding. In vitro, TBBt inhibits different forms of CK2 with Ki values ranging from 80 to 210 nM. TBBz discriminates better between CK2 forms, with Ki values ranging from 70-510 nM. TBBz is more effective than TBBt in inducing apoptosis and to a lesser degree, necrosis in transformed human cell lines. Dvelopment of shRNA strategies for the selective knockdown of the CK2α and CK2α′ isoforms reinforces the foregoing results, indicating that inhibition of CK2 leads to attenuation of proliferation.

Features and Benefits

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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