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T7024 Sigma

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

Synonym: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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Properties

Related Categories Acrylamide, Automated Sequencing, Gel Solutions and Reagents, Molecular Biology, Nucleic Acid Electrophoresis More...
grade   for molecular biology
InChI Key   KWYHDKDOAIKMQN-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99% (GC)
expl. lim.   9.08 %
refractive index   n20/D 1.4179(lit.)
bp   120-122 °C(lit.)
mp   −55 °C(lit.)
density   0.775 g/mL at 20 °C(lit.)
foreign activity   DNase, RNase, protease, none detected

Description

Packaging

25, 50, 100 mL in glass bottle

Quantity

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2372 3 / PGII
WGK Germany 
1
RTECS 
KV7175000
Flash Point(F) 
68 °F
Flash Point(C) 
20 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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