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T7401 Sigma

Triosephosphate Isomerase solution from porcine muscle

Type VII, ammonium sulfate suspension, ~5,000 units/mg protein

Synonym: D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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Properties

type   Type VII
form   ammonium sulfate suspension
foreign activity   3-phosphoglyceric phosphokinase <0.01%
  pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase and aldolase <0.001%
storage temp.   2-8°C

Description

Analysis Note

Protein determined by biuret.

Other Notes

Note: Since the assay of this enzyme seems to be sensitive to minor variables, our packages are now dispensed per mg protein rather than in units.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25°C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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T2507 Triosephosphate Isomerase from baker's yeast (S. cerevisiae), Type I, ammonium sulfate suspension, ~10,000 units/mg protein
T2391 Triosephosphate Isomerase from rabbit muscle, Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

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