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T7428 Sigma

Topoisomerase I from wheat germ

buffered aqueous glycerol solution

Synonym: DNA relaxing enzyme, Topo I

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Properties

Related Categories 5.99.x.x Others, 5.x.x.x Isomerases, Application Index, Biochemicals and Reagents, Cancer Research,
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt 97 kDa
foreign activity   RNase and DNase, none detected
shipped in   dry ice
storage temp.   −70°C

Description

Components

Topoisomerase I is supplied in a solution of 50 mM Tris-HCl, pH 7.9, 1 mM EDTA, 1 mM DTT, 0.5 M NaCl and 50% glycerol (v/v).

Application

Suitable for:
• Analsis of degree of DNA supercoiling
• Forming knots and circles in single stranded DNA
• Converting complementary single stranded DNA to double stranded, circular forms
• Rejoining nicks in double-stranded DNA

Biochem/physiol Actions

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

General description

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closedcircular dsDNA.

Other Notes

To preserve enzyme activity, avoid multiple freeze-thaw cycles and frequent temperature variations.

Unit Definition

One unit will convert 1μg of supercoiled pGEM9Zf(−) plasmid DNA to the relaxed form in 30 min at 37 °C.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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