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T7799 Sigma

Tumor Necrosis Factor-β human

TNF-β, recombinant, expressed in E. coli, powder, cell culture tested

Synonym: TNF-β

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Properties

Related Categories Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Cytokines & Growth Factors, Cytokines and Growth Factors,
recombinant   expressed in E. coli
assay   >95% (SDS-PAGE)
form   powder
potency   4-20 pg/mL Activity
quality   endotoxin tested
mol wt   protein mol wt 18.8 kDa
suitability   cell culture tested
storage temp.   −20°C
Gene Information   human ... LTA(4049), TNFSF13B(10673)

Description

Analysis Note

The biological activity is measured in a cytotoxicity assay using the TNF-suspectible murine L929 cell line in the presence of actinomycin D.

Physical form

lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein

Biochem/physiol Actions

Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Tumor Necrosis Factor (TNF) Superfamily

The tumor necrosis factor (TNF) superfamily comprise an increasing number of structurally related ligand-receptor pairs, including TNF-α, TNF-β (lymphotoxin, LT), TNFR1, TNFR2, LT-βR, osteoprotegrin ...
Jennifer Fries
BioFiles 2009, 4.5, 23.
Keywords: Apoptosis, Biofiles, Cell disruption, Ligands, Microscopy, Type

Peer-Reviewed Papers
15

References

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