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T7941 Sigma

Monoclonal Anti-β-Tubulin IV antibody produced in mouse

clone ONS.1A6, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human, hamster, chicken, bovine, rat, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:400 using cultured human breast adenocarcinoma cell line MCF-7
clone   ONS.1A6, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBB4(10382)
mouse ... Tubb4(22153)
rat ... Tubb4(29213)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

General description

Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have four different isotypes in brain cells, ranging from β I to β IV . Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Monoclonal Anti-β-Tubulin IV antibody is specific for epitope located in the C-terminal sequence of β-tubulin isotype IV in humans, bovines, hamsters, rats, mice and chickens. The product does not react with other tubulin isotypes.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-β-Tubulin IV antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections), indirect ELISA, microarray and western blot (1:400 using cultured human breast adenocarcinoma cell line MCF-7). The product may also be used for immunocytochemistry and immunofluorescence .

Price and Availability


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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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