T8154 Sigma

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

  • CAS Number 72-57-1

  • Empirical Formula (Hill Notation) C34H24N6Na4O14S4

  • Molecular Weight 960.81

  •  Beilstein Registry Number 4360496

  •  MDL number MFCD00003969

  •  PubChem Substance ID 24900505


Frequently Asked Questions

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Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.


Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. It is a vital stain that is not absorbed by healthy viable cells. When cells are damaged or dead, trypan blue can enter the cell allowing dead cells to be counted. The method is sometimes referred to as the dye exclusion method.

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Suggested Laboratory Gloves

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