|Related Categories||In Vivo/In Vitro Tracking of Stem Cells, Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Biology, Cell Culture,|
|suitability||suitable for cell culture|
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Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. It is a vital stain that is not absorbed by healthy viable cells. When cells are damaged or dead, trypan blue can enter the cell allowing dead cells to be counted. The method is sometimes referred to as the dye exclusion method.
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Certificate of Analysis
|Precautionary statements||P201-P308 + P313|
|Personal Protective Equipment||Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||T|
|Risk Statements (Europe)||45|
|Safety Statements (Europe)||53-45|
Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
BioFiles v6 n5, 17–21
Keywords: Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents
For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation
The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions
Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE
Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture
In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors
Nano Cu metal doped on TiO2-SiO2 nanoparticle catalysts in photocatalytic degradation of direct blue dye. Mohamed RM, Mkhalid IA, Al-Thabaiti SA, et al. J. Nanosci. Nanotechnol. 13(7), 4975-80, (2013)
Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks. Iglesias-Guimarais V, Gil-Guiñon E, Sánchez-Osuna M, et al. J. Biol. Chem. 288(13), 9200-15, (2013)
Incision integrity and postoperative outcomes after microcoaxial phacoemulsification performed using 2 incision-dependent systems. Vasavada V, Vasavada AR, Vasavada VA, et al. J. Cataract Refract. Surg. 39(4), 563-71, (2013)
Deciphering the mechanisms of cellular uptake of engineered nanoparticles by accurate evaluation of internalization using imaging flow cytometry. Vranic S, Boggetto N, Contremoulins V, et al. Part. Fibre Toxicol. 10, 2, (2013)
Toll-like receptor 3 activation in retinal pigment epithelium cells - Mitogen-activated protein kinase pathways of cell death and vascular endothelial growth factor secretion. Klettner A, Koinzer S, Meyer T, et al. Acta Ophthalmol. 91(3), e211-8, (2013)
Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Martin-Piedra MA, Garzon I, Oliveira AC, et al. Cytotherapy 15(4), 507-18, (2013)
Effects of light exposure, pH, osmolarity, and solvent on the retinal pigment epithelial toxicity of vital dyes. Costa EF, Barros NM, Coppini LP, et al. Am. J. Ophthalmol. 155(4), 705-12, 712.e1, (2013)
Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels. Gurski LA, Xu X, Labrada LN, et al. PLoS ONE 7(11), e50075, (2012)
Chicago sky blue 6B, a vesicular glutamate transporters inhibitor, attenuates methamphetamine-induced hyperactivity and behavioral sensitization in mice. He Z, Yan L, Yong Z, et al. Behav. Brain Res. 239, 172-6, (2013)
Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth. Petr J, Chmelíková E, Zalmanová T, et al. Animal 7(1), 134-42, (2013)
Retinal function assessment of trypan blue versus indocyanine green assisted internal limiting membrane peeling during macular hole surgery. Bellerive C, Cinq-Mars B, Louis M, et al. Can. J. Ophthalmol. 48(2), 104-9, (2013)
Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation. Fritz JM Mol. Cancer 10, 76, (2011)
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