EMAIL THIS PAGE TO A FRIEND

T8154 Sigma

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

  • CAS Number 72-57-1

  • Empirical Formula (Hill Notation) C34H24N6Na4O14S4

  • Molecular Weight 960.81

  •  Beilstein Registry Number 4360496

  •  MDL number MFCD00003969

  •  PubChem Substance ID 24900505

Purchase

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

Application

Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. It is a vital stain that is not absorbed by healthy viable cells. When cells are damaged or dead, trypan blue can enter the cell allowing dead cells to be counted. The method is sometimes referred to as the dye exclusion method.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells



Learn about PharmaGrade

Additional Products to Explore

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Trypan Blue

powder, BioReagent, suitable for cell culture

0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

TWEEN<SUP>®</SUP> 20

viscous liquid

Customers Also Viewed

Bright-Line<SUP>™</SUP> Hemacytometer

supplied with two cover slips

Trypan Blue

Dye content 60 %

Trypan Blue

powder, BioReagent, suitable for cell culture

Trypan Blue solution

0.4%, for microscopy

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
WGK Germany 
3

Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Determination Of High-affinity Choline Uptake (HACU) And Choline Acetyltransferase (ChAT) Activity In The Same Population Of Cultured Cells. Ray, B., et al. Brain Res. 1297, 160-8, (2009)

Loading...


The tumor suppressor PTEN is exported in exosomes and has phosphatase activity in recipient cells. Putz U, Howitt J, Doan A, et al. Sci. Signal. 5(243), ra70, (2012)

Loading...


Differential binding of inorganic particles to MARCO. Thakur SA Toxicol. Sci. 107(1), 238-46, (2009)

Loading...


Cell specific cytotoxicity and uptake of graphene nanoribbons. Mullick Chowdhury S, Lalwani G, Zhang K, et al. Biomaterials 34(1), 283-93, (2013)

Loading...


Optineurin suppression causes neuronal cell death via NF-κB pathway. Akizuki M, Yamashita H, Uemura K, et al. J. Neurochem. 126(6), 699-704, (2013)

Loading...


Deciphering the mechanisms of cellular uptake of engineered nanoparticles by accurate evaluation of internalization using imaging flow cytometry. Vranic S, Boggetto N, Contremoulins V, et al. Part. Fibre Toxicol. 10, 2, (2013)

Loading...


Human dental pulp stem cells hook into biocoral scaffold forming an engineered biocomplex. Mangano, C PLoS ONE 6(4), e18721, (2011)

Loading...


Femtosecond laser-assisted keratoplasty: full and partial-thickness cut wound strength and endothelial cell loss across a variety of wound patterns. Kopani KR, Page MA, Holiman J, et al. Br. J. Ophthalmol. 98(7), 894-9, (2014)

Loading...


Current concepts of trypan blue in chromovitrectomy. Farah ME, Maia M, Furlani B, et al. Dev. Ophthalmol. 42, 91-100, (2008)

Loading...


Trypan blue dye for anterior segment surgeries. Jhanji V, Chan E, Das S, et al. Eye (Lond.) 25(9), 1113-20, (2011)

Loading...


LYM2-dependent chitin perception limits molecular flux via plasmodesmata. Faulkner C, Petutschnig E, Benitez-Alfonso Y, et al. Proc. Natl. Acad. Sci. U. S. A. 110(22), 9166-70, (2013)

Loading...


Reduced ischemic injury after stroke in mice by angiogenic gene delivery via ultrasound-targeted microbubble destruction. Wang HB, Yang L, Wu J, et al. J. Neuropathol. Exp. Neurol. 73(6), 548-58, (2014)

Loading...


Cauliflower mosaic virus protein P6 inhibits signaling responses to salicylic acid and regulates innate immunity. Love AJ, Geri C, Laird J, et al. PLoS ONE 7(10), e47535, (2012)

Loading...


Cryptococcus neoformans mediator protein Ssn8 negatively regulates diverse physiological processes and is required for virulence. Lin-Ing Wang et al PLoS ONE 6, e19162, (2011)

Loading...


Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks. Iglesias-Guimarais V, Gil-Guiñon E, Sánchez-Osuna M, et al. J. Biol. Chem. 288(13), 9200-15, (2013)

Loading...


Rhof promotes murine marginal zone B cell development. Kishimoto M, Matsuda T, Yanase S, et al. Nagoya J. Med. Sci. 76(3-4), 293-305, (2014)

Loading...


Toll-like receptor 3 activation in retinal pigment epithelium cells - Mitogen-activated protein kinase pathways of cell death and vascular endothelial growth factor secretion. Klettner A, Koinzer S, Meyer T, et al. Acta Ophthalmol. 91(3), e211-8, (2013)

Loading...


Incision integrity and postoperative outcomes after microcoaxial phacoemulsification performed using 2 incision-dependent systems. Vasavada V, Vasavada AR, Vasavada VA, et al. J. Cataract Refract. Surg. 39(4), 563-71, (2013)

Loading...


Desipramine protects neuronal cell death and induces heme oxygenase-1 expression in Mes23.5 dopaminergic neurons. Lin HY, Yeh WL, Huang BR, et al. PLoS ONE 7(11), e50138, (2012)

Loading...


In vitro evidence for mycophenolic acid dose-related cytotoxicity in human retinal cells. Zacharias LC, Damico FM, Kenney MC, et al. Retina (Philadelphia, Pa.) 33(10), 2155-61, (2013)

Loading...


Porcine skin-derived stem cells can serve as donor cells for nuclear transfer. Yanhong Hao et al Cloning Stem Cells 11, 101-10, (2009)

Loading...


A novel assay of antimycobacterial activity and phagocytosis by human neutrophils. Lowe DM, Bangani N, Mehta MR, et al. Tuberculosis (Edinb.) 93(2), 167-78, (2013)

Loading...


Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels. Gurski LA, Xu X, Labrada LN, et al. PLoS ONE 7(11), e50075, (2012)

Loading...


Ophthalmic surgery simulator training improves resident performance of capsulorhexis in the operating room. McCannel CA, Reed DC, and Goldman DR Ophthalmology 120(12), 2456-61, (2013)

Loading...


Chicago sky blue 6B, a vesicular glutamate transporters inhibitor, attenuates methamphetamine-induced hyperactivity and behavioral sensitization in mice. He Z, Yan L, Yong Z, et al. Behav. Brain Res. 239, 172-6, (2013)

Loading...


Isoflurane regulates atypical type-A γ-aminobutyric acid receptors in alveolar type II epithelial cells. Xiang YY, Chen X, Li J, et al. Anesthesiology 118(5), 1065-75, (2013)

Loading...


Electromagnetic field-induced converse cell growth during a long-term observation. Bae JE, Do JY, Kwon SH, et al. Int. J. Radiat. Biol. 89(12), 1035-44, (2013)

Loading...


Effects of light exposure, pH, osmolarity, and solvent on the retinal pigment epithelial toxicity of vital dyes. Costa EF, Barros NM, Coppini LP, et al. Am. J. Ophthalmol. 155(4), 705-12, 712.e1, (2013)

Loading...


Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation. Fritz JM Mol. Cancer 10, 76, (2011)

Loading...


Therapeutic potential of human adipose-derived stem cells (ADSCs) from cancer patients: a pilot study. García-Contreras M, Vera-Donoso CD, Hernández-Andreu JM, et al. PLoS ONE 9(11), e113288, (2014)

Loading...


Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Martin-Piedra MA, Garzon I, Oliveira AC, et al. Cytotherapy 15(4), 507-18, (2013)

Loading...


Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth. Petr J, Chmelíková E, Zalmanová T, et al. Animal 7(1), 134-42, (2013)

Loading...


DLG1 influences distal ureter maturation via a non-epithelial cell autonomous mechanism involving reduced retinoic acid signaling, Ret expression, and apoptosis. Kim ST, Ahn SY, Swat W, et al. Dev. Biol. 390(2), 160-9, (2014)

Loading...


Development of Taenia pisiformis in golden hamster (Mesocricetus auratus). Toral-Bastida E Parasit. Vectors 4, 147, (2011)

Loading...


Inadvertent trypan blue posterior capsule staining during cataract surgery. Burkholder BM, Srikumaran D, Nanji A, et al. Am. J. Ophthalmol. 155(4), 625-628, 628.e1, (2013)

Loading...


Mitochondrial dysfunction in lyssavirus-induced apoptosis. Gholami A J. Virol. 82(10), 4774-84, (2008)

Loading...


Retinal function assessment of trypan blue versus indocyanine green assisted internal limiting membrane peeling during macular hole surgery. Bellerive C, Cinq-Mars B, Louis M, et al. Can. J. Ophthalmol. 48(2), 104-9, (2013)

Loading...


Profiling flavonoid cytotoxicity in human breast cancer cell lines: determination of structure-function relationships. Yadegarynia S, Pham A, Ng A, et al. Nat. Prod. Commun. 7(10), 1295-304, (2012)

Loading...


Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli. Yu G, Jiang L, Xu Y, et al. PLoS ONE 7(11), e48146, (2012)

Loading...


Innovative sugar-insecticide feeding bioassay for adult female Anopheles gambiae (Diptera: Culicidae). Stell FM, Roe RM, Arellano C, et al. J. Med. Entomol. 50(4), 804-15, (2013)

Loading...


Corneal cell adhesion to contact lens hydrogel materials enhanced via tear film protein deposition. Elkins CM, Qi QM, and Fuller GG PLoS ONE 9(8), e105512, (2014)

Loading...


Effects of aflibercept on primary RPE cells: toxicity, wound healing, uptake and phagocytosis. Klettner A, Tahmaz N, Dithmer M, et al. Br. J. Ophthalmol. 98(10), 1448-52, (2014)

Loading...


Dendritic cells pulsed with keyhole limpet hemocyanin and cryopreserved maintain anti-tumor activity in a murine melanoma model. Teitz-Tennenbaum S Clin. Immunol. 129(3), 482-91, (2008)

Loading...


Photodynamic properties of vital dyes for vitreoretinal surgery. Brockmann T, Steger C, and Dawczynski J Ophthalmologica 228(4), 234-8, (2012)

Loading...


Nano Cu metal doped on TiO2-SiO2 nanoparticle catalysts in photocatalytic degradation of direct blue dye. Mohamed RM, Mkhalid IA, Al-Thabaiti SA, et al. J. Nanosci. Nanotechnol. 13(7), 4975-80, (2013)

Loading...


Overexpression of a wheat stearoyl-ACP desaturase (SACPD) gene TaSSI2 in Arabidopsis ssi2 mutant compromise its resistance to powdery mildew. Song N, Hu Z, Li Y, et al. Gene 524(2), 220-7, (2013)

Loading...


Glucosamine suppresses proliferation of human prostate carcinoma DU145 cells through inhibition of STAT3 signaling. Chesnokov, V Cancer Cell Int. 9, 25, (2009)

Loading...


Effect of the steroid K-canrenoate on hsp70 expression and tissue damage in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Siddique YH, Ara G, and Afzal M J. Insect Sci. 12, 92, (2012)

Loading...


Experimental testing of a mathematical model relevant to the extrinsic pathway of apoptosis. Pace V Cell Stress Chaperones 15(1), 13-23, (2010)

Loading...


The discovery of lymphatic stomata and its ultrastructure in mouse tunica vaginalis. Wang J, Wang Z, Yu H, et al. J. Pediatr. Surg. 47(11), 2087-92, (2012)

Loading...


Reply: To PMID 23219064. Weinberg RS, Burkholder BB, Srikumaran D, et al. Am. J. Ophthalmol. 156(5), 1069-70, (2013)

Loading...


Merck 14,9792

Beil. 16,II,255

Related Products

comparable product

Product #

Description

Add to Cart

T6146 Trypan Blue, powder, BioReagent, suitable for cell culture
D2535 Direct Blue 15, suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

recommended

Product #

Description

Add to Cart

Z359629 Bright-Line Hemacytometer, supplied with two cover slips

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?