EMAIL THIS PAGE TO A FRIEND
T8154 Sigma

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

  • CAS Number 72-57-1

  • Empirical Formula (Hill Notation) C34H24N6Na4O14S4

  • Molecular Weight 960.81

  •  Beilstein Registry Number 4360496

  •  MDL number MFCD00003969

  •  PubChem Substance ID 57654704

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Use to detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability and proliferative assays.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

General description

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
Why is Trypan blue, Product T8154, used to determine cell viability?
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
How can I calculate the total number of viable cells in my cell suspension when using Product T8154, Trypan Blue solution?
The calculations for viable cell number can be found on the product insert:http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/t8154pis.pdf
Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?
The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Why are there red fibers in Product T8154, Trypan blue solution?
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Peer-Reviewed Papers
15

References

Related Products

comparable product

Product #

Description

Add to Cart

T6146 Trypan Blue, powder, BioReagent, suitable for cell culture
D2535 Direct Blue 15, suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

recommended

Product #

Description

Add to Cart

Z359629 Bright-Line Hemacytometer, supplied with two cover slips

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?