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  • T8203 - Anti-α-Tubulin antibody, Mouse monoclonal

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T8203 Sigma

Anti-α-Tubulin antibody, Mouse monoclonal

clone AA13, purified from hybridoma cell culture

Synonym: Anti-Tuba1a, Anti-Tubulin, α1A

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   rat, human, mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using Neuro-2a cell extract
clone   AA13, monoclonal
concentration   ~2.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA1A(7846)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat brain tubulin.

General description

Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.
The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.

Antigen Background

Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.

Biochem/physiol Actions

Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).

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