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T8300 Sigma

Anti-Tumor Necrosis Factor-α antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-TNF-α

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   dot blot: 1:2,000
  microarray: suitable
  neutralization: 1-3 μg/mL
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TNF(7124)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

recombinant human TNF-α produced in bacteria.

General description

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody does not react with recombinant human TNF-β, recombinant human IL-1α, recombinant human IL-1β, recombinant human IL-3, recombinant mouse and human IL-6.

Specificity

The antibody binds to TNF-α. It does not cross-react with recombinant human TNF-β, IL-1a, IL-1β or IL-3, nor with recombinant mouse or human IL-6.

Application

Anti-Tumor Necrosis Factor-α antibody may be used for dot blot at a working antibody dilution of 1:2000. The antibody may be used for neutralization assay; the ND50 is 1-3 μg/ml. Anti-TNF-α antibody was used for neutralization of TNF-α of Jurkat cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
15

References

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