|Related Categories||A, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytoskeleton,|
|species reactivity||bovine, human, mouse, rat|
|western blot: 1-2 μg/mL using Neuro-2a cell extract|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|mol wt||antigen mol wt ~50 kDa|
|shipped in||dry ice|
|Gene Information||human ... TUBB(203068)|
purified bovine tubulin
Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β, which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin antibody can be used to visualize HRP signal using enhanced chemiluminescence3. It can also be used in western blotting. Mouse anti-β-tubulin antibody reacts specifically with β tubulin, types I, II, III, and IV of bovine, rat, mouse and human.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Monoclonal anti-β-tubulin antibody (diluted 1: 5000) can be used as a primary antibody in immunofluorescence staining of mammalian oocytes1. It can also be used in numerous immunochemical techniques like-, immunocytochemistry, immunoblotting (50kD approx), immunohistochemistry and immunoprecipitation2.
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clone TUB 2.1, ascites fluid
Certificate of Analysis
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
2. Developmental expression of a neuron-specific beta-tubulin in frog (Xenopus laevis): a marker for growing axons during the embryonic period. Moody, S.A., et al. J. Comp. Neurol. 364, 219-230, (1996)
4. T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules. Lo, W.F., et al. J. Immunol. 162, 5398-5406, (1999)
MicroRNA delivery by cationic lipoplexes for lung cancer therapy. Wu, Y., et al. Mol. Pharmacol. 8, 1381-9, (2011)
The effect of pre-existing maternal obesity on the placental proteome: two-dimensional difference gel electrophoresis coupled with mass spectrometry. Oliva, K., et al. J. Mol. Endocrinol. 48, 139-49, (2012)
Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Ma, P., et al. Proc. Natl. Acad. Sci. U. S. A. 109, E481-9, (2012)
Kaposi's sarcoma-associated herpesvirus latent gene vFLIP inhibits viral lytic replication through NF-kappaB-mediated suppression of the AP-1 pathway: a novel mechanism of virus control of latency. Ye FC J. Virol. 82(9), 4235-49, (2008)
Low plasma membrane expression of the miltefosine transport complex renders Leishmania braziliensis refractory to the drug. María P. Sánchez-Cañete et al Antimicrob. Agents Chemother. 53, 1305-13, (2009)
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