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T8443 Sigma

Trizma® Pre-set crystals

BioPerformance Certified, pH 8.0, average Mw 141.8

Synonym: Tris crystals

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
grade   BioPerformance Certified
mol wt   average Mw 141.8
impurities   DNase, RNase, Protease, NICKase, none detected
  endotoxin, tested
  total aerobic microbial count, tested
  ≤0.05 A290
  ≤0.5% water (Karl Fischer)
  ≤5 ppm Heavy metals (as Pb)
pH   7.71 (37 °C, 0.1 M)(lit.)
  8.00 (25 °C, 0.1 M)(lit.)
  8.0
  8.58 (5 °C, 0.1 M)(lit.)
useful pH range   7.0 - 9.0
suitability   cell culture tested
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Packaging

100, 250 g in poly bottle

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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