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T8512 Sigma

Activated Thiol–Sepharose® 4B

lyophilized powder

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Properties

Related Categories Activated/Functionalized Matrices, Affinity Chromatography, Molecular Biology, Protein Chromatography, Proteomics More...
form   lyophilized powder
extent of labeling   1 μmol per mL
matrix   Sepharose 4B
matrix activation   cyanogen bromide
matrix active group   glutathione 2-pyridyl disulfide
matrix attachment   N-terminal amino group
matrix spacer   10 atoms (when ligands are coupled through the disulfide groups)
swelling   1 g swells to 4-5 mL
storage temp.   2-8°C

Description

Packaging

1, 5 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Activated thiol Sepharose® 4B is used in protein chromatography, affinity chromatography and activated/functionalized matrices. Activated thiol Sepharose® 4B has been used to provide the first report of the isolation of aminopeptidase H from a reptile. Activated thiol Sepharose® 4B has also been used to purify and characterize a neuropeptide-inactivating peptidase.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

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