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T8529 Sigma

Trizma® hydrochloride

for electrophoresis, ≥99%

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

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Properties

grade   for electrophoresis
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
assay   ≥99%
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
cation traces   heavy metals (as Pb): ≤5 ppm
absorption   A40%/290 ≤0.05

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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T5941 Trizma® hydrochloride, BioPerformance Certified, cell culture tested, ≥99.0% (titration)

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