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T8532 Sigma

Triton X-100

for electrophoresis

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
grade   for electrophoresis
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
color   APHA: ≤100
turbidity   ≤5.0 NTU
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: soluble
density   1.06 g/mL at 25 °C(lit.)
HLB   13.5

Description

Application

Detergent used for isoelectric focusing (IEF) and two-dimensional electrophoresis. Concentrations between 1-4% (v/v) are typically used in an IEF gel. Also used for cell permeabilization and protein solubilization including high molecular mass proteins.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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