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  • T8535 - Monoclonal Anti-β-Tubulin I+II antibody produced in mouse

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T8535 Sigma

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse

clone JDR.3B8, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   pig, mouse, rat, chicken, human, bovine
application(s)   indirect ELISA: suitable
  indirect immunofluorescence: suitable
  microarray: suitable
  western blot: 1:1,000 using a chicken fibroblast extract or a chicken brain microtubule proteins preparation
clone   JDR.3B8, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)
rat ... Tubb2(29212), Tubb2c(296554)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA

Specificity

The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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