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T8660 Sigma

Monoclonal Anti-β-Tubulin III antibody produced in mouse

clone SDL.3D10, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Neural Stem Cells,
species reactivity   boar, rat, human, bovine
application(s)   indirect ELISA: suitable
  western blot: 1:400 using a rat brain extract
clone   SDL.3D10, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBB3(10381)
rat ... Tubb3(246118)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA

General description

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.

Specificity

The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2.

Application

Monoclonal Anti-β-Tubulin III antibody produced in mouse has been used in following studies:
• Immunocytochemistry.
• Flow cytometry.
• Immunoblotting.

Biochem/physiol Actions

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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Description

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T3952 Anti-β-Tubulin III antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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