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T8778 Sigma

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym: α,α-Trehalose glucohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
assay   0.5-10.0 mg/mL protein basis (BCA)
form   buffered aqueous glycerol solution
foreign activity   α-galactosidase, invertase and α-glucosidase ≤1%
  β-glucosidase ≤5%
  amylase ≤2%
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton X-100 and 25 mM potassium phosphate, pH 6.5

Application

Trehalase has been used in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves. Trehalase has also been used in a study to investigate growth arrest by trehalose-6-phosphate.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How is Product T8778, Trehalase from porcine kidney prepared?
The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).
What is the molecular weight of Product T8778, Trehalase from porcine kidney?
The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979). 
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References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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