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T8778 Sigma

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym: α,α-Trehalose glucohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
assay   0.5-10.0 mg/mL protein basis (BCA)
form   buffered aqueous glycerol solution
foreign activity   α-galactosidase, invertase and α-glucosidase ≤1%
  β-glucosidase ≤5%
  amylase ≤2%
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton X-100 and 25 mM potassium phosphate, pH 6.5

Application

Trehalase has been used in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves. Trehalase has also been used in a study to investigate growth arrest by trehalose-6-phosphate.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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