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T8787 Sigma

Triton X-100

for molecular biology

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Apoptosis and Cell Cycle, Biochemicals, Biochemicals and Reagents, Cell Biology,
grade   for molecular biology
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: soluble0.1 mL/mL, clear, colorless to faintly yellow
density   1.06 g/mL at 25 °C(lit.)
HLB   13.5
foreign activity   DNase and RNase, none detected

Description

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

50, 100, 250 mL in poly bottle

Application

Triton X-100 has been used:-
• To permeabilise cells during Immunofluorescent microscopic studies
• In immunohistochemistry for staining the Flat-mount retinas
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure

General description

Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Protocols

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Peer-Reviewed Papers
15

References

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