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T8787 Sigma

Triton X-100

for molecular biology

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Apoptosis and Cell Cycle, Biochemicals, Biochemicals and Reagents, Cell Biology,
grade   for molecular biology
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   water: soluble0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
density   1.06 g/mL at 25 °C(lit.)
HLB   13.5
foreign activity   DNase and RNase, none detected

Description

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

50, 100, 250 mL in poly bottle

Application

Triton X-100 has been used:-
• To permeabilise cells during Immunofluorescent microscopic studies
• In immunohistochemistry for staining the Flat-mount retinas
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure

General description

Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Protocols

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Peer-Reviewed Papers
15

References

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