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T9026 Sigma

Monoclonal Anti-α-Tubulin antibody produced in mouse

clone DM1A, ascites fluid

Synonym: Monoclonal Anti-α-Tubulin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
application(s)   indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
  western blot: 1:500 using human or chicken fibroblasts
clone   DM1A, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

microtubules from chicken embryo brain.

General description

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).

Price and Availability


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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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