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  • T9028 - Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

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T9028 Sigma

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

clone TUB-1A2, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   plant, animal, human
application(s)   indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
  microarray: suitable
  western blot: suitable
clone   TUB-1A2, monoclonal
antibody form   ascites fluid
isotype   IgG3
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA4A(7277), TUBB(203068)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

peptide containing the carboxy-terminal amino acids of α-tubulin

General description

The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Specificity

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.

Application

Monoclonal anti-tubulin tyrosine antibody can be used in indirect immunofluorescence and immunoblotting.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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