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T9069 Sigma

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

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Properties

Related Categories 5.99.x.x Others, 5.x.x.x Isomerases, Application Index, Biochemicals and Reagents, Cancer Research,
assay   ≥2 units/μL
form   buffered aqueous glycerol solution
mol wt   mol wt 100 kDa
storage temp.   −70°C
Gene Information   human ... TOP1(7150)

Description

Biochem/physiol Actions

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

General description

Cellular topoisomerase I is present in retroviral particles and enhances viral cDNA synthesis.

Unit Definition

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

Physical form

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

Application

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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