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T9128 Sigma

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

Synonym: SBTI

Description

Frequently Asked Questions

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Components

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Application

This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

Caution

A solution at 10 mg/mL should be stored for greater than 3 years at 2-8°C retained activity. Solutions are stable in frozen aliquots at -20°C.

Preparation Note

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Unit Definition

One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C. One mg will inhibit 1.0-3.0 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

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Trypsin inhibitor from <I>Glycine max</I> (soybean)

Type I-S, lyophilized powder

Trypsin inhibitor from <I>Glycine max</I> (soybean)

powder, BioReagent, suitable for cell culture

Trypsin inhibitor from <I>Glycine max</I> (soybean)

solution, 1 ×, sterile-filtered, BioReagent, suitable for cell culture

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