T9128 Sigma

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

Synonym: SBTI

Description

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Analysis Note

One mg will inhibit minimum 1.0 mg of trypsin with activity of approx.10,000 BAEE units per mg protein.

Other Notes

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Preparation Note

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Biochem/physiol Actions

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.

General description

Soybean trypsin inhibitor exhibits a high degree of sequence homology with cathepsin D inhibitor from potatoes.1

Price and Availability

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