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T9157 Sigma

Tryptose Phosphate Broth

insect cell culture tested

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Related Categories Bacterial Culture Media, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology, Tryptose Broth
suitability   insect cell culture tested

Description

Components

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Packaging

100, 500 g in poly bottle

Application

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.

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Safety Information

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
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Description

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T8159 Tryptose Phosphate Broth solution, sterile-filtered, suitable for cell culture
T8782 Tryptose Phosphate Broth, buffered powder, Microbiologically tested.
T4532 Tryptose Broth, insect cell culture tested

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