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T9194 Sigma

Topoisomerase I from vaccinia virus

buffered aqueous glycerol solution

Synonym: TOPO® I

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Description

Application

Enzyme activity is increased in the presence of 2.5 mM Mg2+.

Topoisomerase I from vaccinia virus can be used for studying pivotal biological process such as-replication, transcription, recombination as well as DNA structure and topology which includes chromatin reconstitution in vitro and the degree of supercoiling of DNA. Additionally, the product helps in relaxing the DNA coils and exposes the restriction sites which facilitates in enhancing the restriction endonuclease digestion of resistant DNA. It is also used for assaying mutant plasmids which differ in length by only one base-pair.

Biochem/physiol Actions

Topoisomerase I from vaccinia virus also refers as TOPO ®I is a type I DNA topoisomerase, which cleaves DNA at the preferred sequence [5′(C/T)CCTTI]. The product assists in releasing the supercoiling and torsional tension of DNA by cleaving and religating the phosphodiester bonds in a single strand of DNA.

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Unit Definition

One unit coverts 1 μg of supercoiled closed circular (Form I) pUC19 DNA to relaxed closed circular form (Form II) in 1 hr at 37 °C.

Physical form

Solution in 50 mM Tris HCl, pH 7.5, containing 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and 50% glycerol.

Legal Information

TOPO is a registered trademark of Life Technologies

Price and Availability


All labs need water
Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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