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T9281 Sigma

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, suitable for electrophoresis, ~99%

Synonym: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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Description

Analysis Note

Tested as a catalyst for PAGE.

Caution

Handle and store under inert gas, air and moisture sensitive.

Packaging

25, 50, 100 mL in glass bottle

Application

TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.

General description

TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.

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Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2372 3 / PGII
WGK Germany 
1
RTECS 
KV7175000
Flash Point(F) 
68 °F
Flash Point(C) 
20 °C
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Peer-Reviewed Papers
15

References

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