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T9285 Sigma

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym: TE buffer solution

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
InChI Key   VLEIUWBSEKKKFX-UHFFFAOYSA-N
sterility   0.2 μm filtered
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Application

Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.
• Tris-EDTA buffer solution has been used in culture medium.
• Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.
• It has been used for the suspension of bacterial spores.
• It has also been used as a buffer in immunohistochemistry.

General description

TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?
When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.
What is the pH and color of Product T9285, Tris-EDTA buffer solution?
The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis. Please see the attached specification sheet link: http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symbol=T9285
What is the shelf life of Product T9285, Tris-EDTA buffer solution?
This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis. Please see the following link to the Product Dating and Shipping document, which is an Adobe Acrobat Reader .pdf file: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/product-dating-information-statementv4.pdf
What does 'for molecular biology' mean?
The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note

Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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