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T9285 Sigma

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym: TE buffer solution



Related Categories Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
sterility   0.2 μm filtered
foreign activity   DNase, RNase, none detected
storage temp.   room temp


Frequently Asked Questions

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TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.


Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks. Tris-EDTA buffer solution is used in culture medium.1,2

General description

TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

Price and Availability

Synthetic Biology Resource Collection

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations


Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note

Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

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