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T9424 Sigma

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

Synonym: TRI Reagent® RNA Isolation Reagent

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

The reagent has been used for isolating RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.

Features and Benefits

• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods

Packaging

25, 100, 200 mL in glass bottle

General description

TRI Reagent is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein. Successful isolations from human, animal, plant, yeast, bacterial, and viral samples can be obtained. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an improvement of the single-step method reported by Chomczynski and Sacchi for total RNA isolation. TRI Reagent performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One ml of TRI Reagent is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 ´ 106 cells, or 10 cm2 of culture dish surface for cells grown in monolayer.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Price and Availability


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Safety & Documentation

Safety Information

Signal word 
Danger
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 2821 6.1 / PGIII
WGK Germany 
2
Flash Point(F) 
174.2 °F
Flash Point(C) 
79 °C

Frequently Asked Questions

My cells are plated on polystyrene culture plates. How do I use Product T9424, TRI Reagent in this application?
We recommend harvesting the cells in PBS with a rubber policeman/cell scraper, transfer to an eppendorf tube, and proceed with the RNA isolation by removing the PBS from the cell pellet and re-suspending the pellet in the Tri Reagent.
If I add a volume of Product T9424, Tri Reagent™, different from 1 ml, how much chloroform and isopropanol should I add?
What is important is to keep the ratio the same. For example, we recommend adding 200 µL of chloroform and 500 µL of isopropanol when using 1 ml of Tri Reagent. Therefore, 100 µL of chloroform and 250 µL of isopropanol would be added if using 500 µL of Tri Reagent. Similarly, 300 µL of chloroform and 750 µL of isopropanol would be added to if using 1.5 ml of Tri Reagent.
What is the expiration date for Product T9424, Tri Reagent™?  
The expiration date for T9424 has not been established. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt. Click on the following link to view Shipping/Storage/Shelf Life/Expiration date statement <rn:answer_xref style="TEXT-INDENT: 0in" title="Product Dating" target="_self" contents="http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/General_Information/product-dating-information-statement-v5.pdf" answer_id="53" />
Can I use T9424 Tri Reagent™ for blood samples?
No, we recommend using T3809 Tri Reagent BD for work in blood samples.
Which chloroform and isopropanol do you recommend to be used with T9424, Tri Reagent™?
For chloroform and isopropanol we recommend C2432 and I9616, respectively.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Cultrex® 3-D Culture Cell Harvesting Kit Protocol

3D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of ...
Keywords: Buffers, Cell culture, Cell disruption, Cell proliferation, Culture media, Diagnostic, Western blot

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

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