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T9525 Sigma

Tris-Borate-EDTA buffer

working solution

Synonym: TBE buffer

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z More...
InChI Key   OSBLTNPMIGYQGY-UHFFFAOYSA-N
sterility   0.2 μm filtered
form   working solution
impurities   DNase, RNase, Protease, none detected
suitability   suitable for gel electrophoresis (after dilution to working concentration)
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Other Notes

89 mM Tris borate, pH approx. 8.3, containing 2 mM EDTA.

Packaging

Supplied in dispenser with a spigot.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Solution prepared with 18 megohm water

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers
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