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T9691 Sigma

Anti-phospho-TrkA (pTyr490) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   rat, mouse, human
application(s)   immunocytochemistry: 1:100 using cultured cells
  immunohistochemistry (frozen sections): 1:10
  immunoprecipitation: 1:250
  western blot: 1:1,000 using NGF-treated PC12 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 140 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... NTRK1(4914)
mouse ... Ntrk1(18211)
rat ... Ntrk1(59109)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide Tyr490 corresponding to residues surrounding Tyr490 of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC.

General description

TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr490, Tyr674, Tyr675, Tyr751, and Tyr785 and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr490) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr490 in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr490) antibody is reactive in mouse, human and rat.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol.

Application

Anti-phospho-TrkA (pTyr490) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells.

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RIDADR  NONH for all modes of transport
WGK Germany  2
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