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TDF100A Sigma

Total Dietary Fiber Assay Kit

sufficient for ~100 assays

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Description

Other Notes

Reagents for the determination of total dietary fiber.

Principle

Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).

Application

This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.

Price and Availability


All labs need water

Standard component

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α-Amylase, Heat-stable 10 mL SDS    
Amyloglucosidase 10 mL SDS    
Celite 50 g SDS    
Protease 500 mg SDS    
Safety & Documentation
Protocols & Articles

Articles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

Peer-Reviewed Papers
15

References

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