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TOX6 Sigma

In Vitro Toxicology Assay Kit, Sulforhodamine B based

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

For spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. Absorbance of dye is measured at a wavelength of 565 nm.

Biochem/physiol Actions

Dye binds to cellular protein and is then solubilized in base.

Price and Availability


Flow Cytometry Instruments

Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed.  This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture. The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension.  Otherwise, you need to centrifuge, remove the media and then fix.  Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.  
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Peer-Reviewed Papers
15

References

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