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U0631 Sigma

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Synonym: Carbamide, Carbonyldiamide

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Properties

Related Categories Biochemicals and Reagents, Building Blocks, Carbonyl Compounds, Chaotropic Agents, Chemical Synthesis,
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
product line   BioXtra
assay   99.0-100.5%
form   powder
impurities   ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
pH   7.5-9.5 (20 °C, 5 M in H2O)
mp   132-135 °C(lit.)
solubility   H2O: soluble5 M at 20 °C, clear, colorless
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%
absorption   A5M/260 nm ≤0.06
  A5M/280 nm ≤0.06
Featured Industry   Diagnostic Assay Manufacturing
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

1 kg in poly bottle

5 kg in poly drum

500 g in poly bottle

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles
Peer-Reviewed Papers
15

References

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