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U3254 Sigma

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

clone DECMA-1, ascites fluid, buffered aqueous solution

Synonym: Anti-E-Cadherin, Anti-LCAM

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human, bovine, canine, mouse
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect immunofluorescence: 1:1,600 using cultured MDCK cells
  microarray: suitable
  western blot: suitable
clone   DECMA-1, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDH1(999)
mouse ... Cdh1(12550)
biological source   rat
conjugate   unconjugated

Description

Immunogen

mouse embryonal carcinoma cell line PCC4 Aza R1.

General description

Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein uvomorulin, initially identified in embryonal carcinoma, is identical to E-Cadherin, L-CAM, Cell CAM 80/120, and ARC-1, each of which have been characterized in different systems. Uvomorulin/E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.

Specificity

Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays.

Application

Monoclonal Anti-Uvomorulin/E-Cadherin antibody may be used for immunofluorescent labeling of uvomorulin/E-C adherin on confluent cell layers grown in culture. The antibody can also be used in immunoblotting and immunoprecipitation techniques with mouse or dog tissue. It can be used for studies of embryonal development, cell-cell interation of cells grown in culture, and localization of uvomorulin/E-Cadherin (L-CAM).

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RIDADR  NONH for all modes of transport
WGK Germany  2
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