Uvomorulin/E-Cadherin monoclonal antibody | Immunohistochemistry, Western, Immunofluorescence, Immunoprecipitation | U3254

Properties

Related Categories	Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Hematopoietic Stem Cells, Antibodies for Pluripotent Stem Cells, Antibodies for Stem Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cytoskeleton, Antibodies to Extracellular Matrix/Cytoskeleton, Cell Biology, Clone Index, D, General Stem Cell Biology, Hematopoietic Stem Cell Antibodies, Hematopoietic Stem Cell Biology, Primary Antibodies, Stem Cell Biology, Stem Cell Characterization, Stem Cell Differentiation, UH-UZ More...
species reactivity	human, bovine, canine, mouse
application(s)	immunohistochemistry (frozen sections): suitable
	immunoprecipitation: suitable
	indirect immunofluorescence: 1:1,600 using cultured MDCK cells
	microarray: suitable
	western blot: suitable
clone	DECMA-1, monoclonal
antibody form	ascites fluid
form	buffered aqueous solution
isotype	IgG1
contains	15 mM sodium azide
shipped in	dry ice
storage temp.	−20°C
Gene Information	human ... CDH1(999) mouse ... Cdh1(12550)

Description

Immunogen

mouse embryonal carcinoma cell line PCC4 Aza R1.

General description

Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein uvomorulin, initially identified in embryonal carcinoma, is identical to E-Cadherin, L-CAM, Cell CAM 80/120, and ARC-1, each of which have been characterized in different systems. Uvomorulin/E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca²⁺.

Specificity

Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays.

Application

Monoclonal Anti-Uvomorulin/E-Cadherin antibody may be used for immunofluorescent labeling of uvomorulin/E-C adherin on confluent cell layers grown in culture. The antibody can also be used in immunoblotting and immunoprecipitation techniques with mouse or dog tissue. It can be used for studies of embryonal development, cell-cell interation of cells grown in culture, and localization of uvomorulin/E-Cadherin (L-CAM).