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U5378 Sigma

Urea

powder, BioReagent, for molecular biology, suitable for cell culture

Synonym: Carbamide, Carbonyldiamide

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
grade   for molecular biology
product line   BioReagent
assay   ≥98%
form   powder
color   white
mp   132-135 °C(lit.)
solubility   H2O: soluble480 mg/mL, clear, colorless (8M)
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤5 ppm
cation traces   Cu: ≤1 ppm
  Fe: ≤1 ppm
  Pb: ≤2 ppm
absorption   A6M/260 ≤0.06
  A6M/280 ≤0.06
suitability   suitable for cell culture
foreign activity   DNase, NICKase, RNase and protease, none detected
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles

Articles

Determination of Water Content in Urea Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Peer-Reviewed Papers
15

References

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