U5378 Sigma


powder, BioReagent, for molecular biology, suitable for cell culture

Synonym: Carbamide, Carbonyldiamide



Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
grade   for molecular biology
product line   BioReagent
form   powder
color   white
mp   132-135 °C(lit.)
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤5 ppm
cation traces   Cu: ≤1 ppm
  Fe: ≤1 ppm
  Pb: ≤2 ppm
absorption   A6M/260 ≤0.06
  A6M/280 ≤0.06
suitability   suitable for cell culture
foreign activity   DNase, NICKase, RNase and protease, none detected
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)



Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.


1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

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Safety & Documentation
Protocols & Articles


Determination of Water Content in Urea Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

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