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U5379 Sigma

Anti-Ubiquitin antibody produced in rabbit

whole antiserum, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Protein Degradation, Antibodies to Transcription Factors,
species reactivity   eukaryotes, prokaryotes
application(s)   immunohistochemistry (frozen sections): suitable
  western blot: 1:100 using ubiquitin purified from bovine red blood cells
clone   polyclonal
antibody form   whole antiserum
form   lyophilized powder
mol wt   antigen mol wt 8.6 kDa
storage temp.   2-8°C
Gene Information   human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

bovine red blood cell ubiquitin.

Antigen Background

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

Physical form

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

Specificity

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

Reconstitution

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

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