U5382 Sigma

Ubiquitin human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), essentially salt-free, lyophilized powder

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in E. coli (N-terminal FLAG® tagged)
assay   ≥95% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
mol wt   mol wt 10 kDa
solubility   0.05 M Tris pH 7.5: ≥10 mg/mL
storage temp.   −20°C
Gene Information   human ... USP10(9100)

Description

Application

Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.1

Packaging

Package size based on protein content

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

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