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U6258 Sigma

UbcH9 Conjugating Enzyme human

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Properties

recombinant   expressed in baculovirus infected Sf9 cells
assay   ≥98% (SDS-PAGE)
form   aqueous glycerol solution
mol wt   mol wt 20 kDa
shipped in   dry ice
storage temp.   −70°C

Description

Application

Typical enzyme concentration to support in vitro conjugation is 100nM to 1μM dependent upon conditions.

Biochem/physiol Actions

UbcH9 mediates the conjugation of SUMO-1, SUMO-2, and SUMO-3 to a diverse variety of proteins without the help of an E3 ligase. This E2 conjugating enzyme interacts directly with protein substrates that are modified by SUMOylation, and may help in substrate recognition. UbcH9 is specific for the conjugation of proteins modified by SUMOylation.

Physical form

Supplied at a concentration of 0.5 μg/μl in a solution of 50mM HEPES, pH 7.6, 125 mM NaCl, 10% glycerol, and 1 mM DTT.

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Safety Information

Documents

Certificate of Analysis

Protocols & Articles
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U9257 UbcH9 Conjugating Enzyme human, ≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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