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U6382 Sigma

Anti-UCP-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Uncoupling Protein 1

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Obesity Research, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue.
  western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~32 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... UCP1(7350)
mouse ... Ucp1(22227)
rat ... Ucp1(24860)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1.

General description

Mitochondrial oxidative phosphorylation makes ATP synthesis possible using the energy available from substrate oxidation at the respiratory chain. These processes are coupled through the proton electrochemical potential gradient generated during the transfer of electrons from the substrate to oxygen. The uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that are considered to be transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They are capable of returning protons pumped by the respiratory chain to the mitochondrial matrix.
Uncoupling proteins currently comprise UCP-1, -2, -3, -4, and -5. UCP-1 is a 32 kDa protein that is active as a proton channelforming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids. Proton transport by UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor. UCP-1 is exclusively expressed in BAT in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. Stimulated BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration. The liberated heat can serve several physiological functions, e.g., for body heating during emergence from hibernation or during cold exposure, for burning body fat and consequently for body weight regulation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding.

Application

Anti-UCP-1 polyclonal antibody is suitable for detection of UCP-1 by immunochemical methods such as immunohistochemistry and immunoblotting (~32 kDa). Additional weak bands may be detected in some preparations of brown adipose tissue (BAT) extracts by immunoblotting.
Immunoblotting: a minimum working antibody dilution of 1:1,000 is determined using an extract of rat brown adipose tissue mitochondria.
Immunohistochemistry: a minimum working antibody dilution of 1:500 is determined using protease-digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.

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