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U6504 Sigma

Urea

for electrophoresis

Synonym: Carbamide, Carbonyldiamide

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Properties

Related Categories Biochemicals and Reagents, Buffers and Reagents, Buffers, Reagents, & Stains, Chaotropic Agents, Core Bioreagents,
grade   for electrophoresis
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
assay   ≥98%
form   powder or crystals
impurities   ≤0.005% Ammonia
mp   132-135 °C(lit.)
solubility   H2O: soluble100 mg/mL
density   1.335 g/mL at 25 °C(lit.)
cation traces   Fe: ≤1 ppm
  Pb: ≤5 ppm
absorption   A5M/260 ≤0.06
  A5M/280 ≤0.06
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

1 kg in poly bottle

500 g in poly bottle

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles
Peer-Reviewed Papers
15

References

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