Urea
for electrophoresis
DOWNLOAD MSDS (PDF)Synonym: Carbamide, Carbonyldiamide
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CAS Number 57-13-6
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Linear Formula NH2CONH2
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Molecular Weight 60.06
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Beilstein Registry Number 635724
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EC Number 200-315-5
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MDL number MFCD00008022
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PubChem Substance ID 24900435
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Popular Documents: Product Information Sheet (PDF) | Specification Sheet (PDF) | RAMAN FTIR (PDF)
Properties
| Related Categories | Bioactive Small Molecules, Biochemicals and Reagents, Buffers and Reagents, Buffers, Reagents, & Stains, Cell Biology, |
| grade | for electrophoresis |
| impurities | ≤0.005% Ammonia |
| mp | 132-135 °C(lit.) |
| density | 1.335 g/mL at 25 °C(lit.) |
| cation traces | Fe: ≤1 ppm |
| Pb: ≤5 ppm | |
| abs. |
A |
Description
Application
A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
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