U6756 Sigma

U-73122 hydrate


Synonym: 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione



Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
form   powder
color   off-white
solubility   ethanol: ~.7 mg/mL
  DMSO: ~.9 mg/mL
  chloroform: ~10 mg/mL
  DMSO: ≤2.6 mg/mL (Achieved with heating.)
  ethanol: ≤5 mg/mL (Achieved with heating.)
  H2O: insoluble
storage temp.   room temp


Biochem/physiol Actions

Inhibits the hydrolysis of PPI to IP3, which leads to a decrease in cytosolic free calcium. Inhibits the coupling of G protein-phospholipase C activation, while remaining unaffected by production of cAMP.

Preparation Note

U-73122 hydrate is soluble in ethanol (approx. 0.7 mg/ml or ≤ 5 mg/ml with heating), chloroform (approx. 10 mg/ml) and DMSO (approx. 0.9 mg/ml or ≤ 2.6 mg/ml with heating). U-73122 hydrate is insoluble in water.


U-73122 hydrate has been used for studying the role of phospholipase C (PLC) signaling in modulating epithelial Na+ channel (ENaC) function in gerbil Reissner′s membranes (RMs)1. U-73122 has also been used to study local calcium responses in human umbilical vein endothelial cells (HUVECs)2.

Features and Benefits

This compound is featured on the Phospholipase C page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Price and Availability

DISCOVER Bioactive Small Molecules

Customers Also Viewed


>97% (HPLC)

RHC 80267

≥98% (HPLC), solid


≥90%, powder

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 

Protocols & Articles


Discover Bioactive Small Molecules for Lipid Signaling Research

Within mammalian cells, a multitude of lipid compounds are found with a variety of cellular functions, including structural components of cell membranes and as second messengers in cell signaling pat...
Keywords: Absorption, Atomic absorption spectroscopy, Cancer, Cardiovascular, Cell signaling, Diabetes, Digestions, Diseases, Growth factors, Hormones, Inflammation, Lipid Metabolism, Lipid signaling, Metabolism

The Role of Inflammation in Neurodegeneration

It is well established that the brain can sense and react to peripheral insults through the rapid induction of fever and other neuroendocrine changes in response to events such as infection and tissu...
Scott Hauser, Technology Transfer Specialist, Sigma® Life Science
Biofiles, Vol. 8, No. 19
Keywords: Adhesion, Central Nervous System, Diseases, Gene expression, Inflammation, Neurodegenerative Diseases, Reductions

Peer-Reviewed Papers


Set your institution to view full text papers.

1. P2Y4-mediated Regulation Of Na+ Absorption In The Reissner's Membrane Of The Cochlea. Kim, C.H., et al. J. Neurosci. 30, 3762-9, (2010)


2. Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels. Nishitani WS, Saif TA, and Wang Y PLoS ONE 6(10), e26181, (2011)


Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils. Bleasdale, et al. J. Pharmacol. Exp. Ther. 255, 756, (1990)


U-73122, an aminosteroid phospholipase C antagonist, noncompetitively inhibits thyrotropin-releasing hormone effects in GH3 rat pituitary cells. Smallridge, et al. Endocrinology 131, 1883-1888, (1992)


U73122 inhibits Ca2+ oscillations in response to cholecystokinin and carbachol but not to JMV-180 in rat pancreatic acinar cells. Yule, et al. J. Biol. Chem. 267, 13830-13835, (1992)


Lysophosphatidylcholine Protects Cerebellar Granule Neurons From Apoptotic Cell Death. Ikeno, Y., et al. J. Neurosci. Res. 87, 190-9, (2009)


Helicobacter pylori γ-glutamyl transpeptidase-induced Ca(2+) release via PLC-IP3 receptors in AGS cells. Park EH, Kim JM, Kim KM, et al. Can. J. Microbiol. 60(12), 865-8, (2014)


Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. Chang KY, Woo D, Jung H, et al. Nat. Commun. 5, 4057, (2014)

Losartan, an angiotensin II type 1 receptor blocker, protects human islets from glucotoxicity through the phospholipase C pathway. Madec AM, Cassel R, Dubois S, et al. FASEB J. 27(12), 5122-30, (2013)


In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium. Aihara E, Hentz CL, Korman AM, et al. J. Biol. Chem. 288(47), 33585-97, (2013)


Superpriming of synaptic vesicles after their recruitment to the readily releasable pool. Lee JS, Ho WK, Neher E, et al. Proc. Natl. Acad. Sci. U. S. A. 110(37), 15079-84, (2013)


Olfactory transduction pathways in the Senegalese sole Solea senegalensis. Velez Z, Hubbard PC, Barata EN, et al. J. Fish Biol. 83(3), 501-14, (2013)


The role of intracellular pathways in the proliferation of human K562 cells mediated by muscarinic receptors. Aydın B, Kan B, and Cabadak H Leuk. Res. 37(9), 1144-9, (2013)


Calcium release from intra-axonal endoplasmic reticulum leads to axon degeneration through mitochondrial dysfunction. Villegas R, Martinez NW, Lillo J, et al. J. Neurosci. 34(21), 7179-89, (2014)


Calcitonin controls bone formation by inhibiting the release of sphingosine 1-phosphate from osteoclasts. Keller J, Catala-Lehnen P, Huebner AK, et al. Nat. Commun. 5, 5215, (2014)


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?