U6756 Sigma

U-73122 hydrate


Synonym: 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione



Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
form   powder
color   off-white
solubility   ethanol: ~.7 mg/mL
  DMSO: ~.9 mg/mL
  chloroform: ~10 mg/mL
  DMSO: ≤2.6 mg/mL (Achieved with heating.)
  ethanol: ≤5 mg/mL (Achieved with heating.)
  H2O: insoluble
storage temp.   room temp


Biochem/physiol Actions

Inhibits the hydrolysis of PPI to IP3, which leads to a decrease in cytosolic free calcium. Inhibits the coupling of G protein-phospholipase C activation, while remaining unaffected by production of cAMP.

Preparation Note

U-73122 hydrate is soluble in ethanol (approx. 0.7 mg/ml or ≤ 5 mg/ml with heating), chloroform (approx. 10 mg/ml) and DMSO (approx. 0.9 mg/ml or ≤ 2.6 mg/ml with heating). U-73122 hydrate is insoluble in water.


U-73122 hydrate has been used for studying the role of phospholipase C (PLC) signaling in modulating epithelial Na+ channel (ENaC) function in gerbil Reissner′s membranes (RMs)1. U-73122 has also been used to study local calcium responses in human umbilical vein endothelial cells (HUVECs)2.

Features and Benefits

This compound is featured on the Phospholipase C page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Price and Availability

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Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 
Protocols & Articles


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Peer-Reviewed Papers


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1. P2Y4-mediated Regulation Of Na+ Absorption In The Reissner's Membrane Of The Cochlea. Kim, C.H., et al. J. Neurosci. 30, 3762-9, (2010)


2. Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels. Nishitani WS, Saif TA, and Wang Y PLoS ONE 6(10), e26181, (2011)


Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils. Bleasdale, et al. J. Pharmacol. Exp. Ther. 255, 756, (1990)


U-73122, an aminosteroid phospholipase C antagonist, noncompetitively inhibits thyrotropin-releasing hormone effects in GH3 rat pituitary cells. Smallridge, et al. Endocrinology 131, 1883-1888, (1992)


U73122 inhibits Ca2+ oscillations in response to cholecystokinin and carbachol but not to JMV-180 in rat pancreatic acinar cells. Yule, et al. J. Biol. Chem. 267, 13830-13835, (1992)


Lysophosphatidylcholine Protects Cerebellar Granule Neurons From Apoptotic Cell Death. Ikeno, Y., et al. J. Neurosci. Res. 87, 190-9, (2009)


Coronin-1 and calcium signaling governs sympathetic final target innervation. Suo D, Park J, Young S, et al. J. Neurosci. 35(9), 3893-902, (2015)


Helicobacter pylori γ-glutamyl transpeptidase-induced Ca(2+) release via PLC-IP3 receptors in AGS cells. Park EH, Kim JM, Kim KM, et al. Can. J. Microbiol. 60(12), 865-8, (2014)


Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. Chang KY, Woo D, Jung H, et al. Nat. Commun. 5, 4057, (2014)

Losartan, an angiotensin II type 1 receptor blocker, protects human islets from glucotoxicity through the phospholipase C pathway. Madec AM, Cassel R, Dubois S, et al. FASEB J. 27(12), 5122-30, (2013)


In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium. Aihara E, Hentz CL, Korman AM, et al. J. Biol. Chem. 288(47), 33585-97, (2013)


Superpriming of synaptic vesicles after their recruitment to the readily releasable pool. Lee JS, Ho WK, Neher E, et al. Proc. Natl. Acad. Sci. U. S. A. 110(37), 15079-84, (2013)


Olfactory transduction pathways in the Senegalese sole Solea senegalensis. Velez Z, Hubbard PC, Barata EN, et al. J. Fish Biol. 83(3), 501-14, (2013)


The role of intracellular pathways in the proliferation of human K562 cells mediated by muscarinic receptors. Aydın B, Kan B, and Cabadak H Leuk. Res. 37(9), 1144-9, (2013)


Calcium release from intra-axonal endoplasmic reticulum leads to axon degeneration through mitochondrial dysfunction. Villegas R, Martinez NW, Lillo J, et al. J. Neurosci. 34(21), 7179-89, (2014)


Calcitonin controls bone formation by inhibiting the release of sphingosine 1-phosphate from osteoclasts. Keller J, Catala-Lehnen P, Huebner AK, et al. Nat. Commun. 5, 5215, (2014)


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