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U7757 Sigma

Anti-UCP-3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Obesity Research, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using protease-digested sections of human and mouse skeletal muscle.
  western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue and extract of E. coli expressing UCP3
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~34 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... UCP3(7352)
mouse ... Ucp3(22229)
rat ... Ucp3(25708)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids, respectively.

synthetic peptide corresponding to human UCP-3 sequence (amino acids 254-267) with N-terminal lysine added, conjugated to KLH.

General description

UCP3 is a skeletal muscle protein that functions to uncouple mitochondrial respiration from ATP production. UCP3 regulates fatty acid and energy metabolism. This protein inhibits lipid-induced oxidative stress in the mitochondrial components of heart and skeletal muscle cells. UCP3 may also be involved in the pathogenesis of type 2 diabetes . Anti-UCP-3 antibody is specific for UCP3 in humans, rats and mice and does not react with UCP1 and UCP2. Staining of the UCP3 band by immunoblotting is specifically inhibited with the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Application

Anti-UCP-3 antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections at 1:3,000 dilution, using protease-digested sections of human and mouse skeletal muscle). The antibody may also be used in immunoblot (approx. 34 kDa, additional weak bands may be detected in some preparations of brown adipose tissue extracts) and western blot (at 1:1,000 dilution, using mitochondrial extract of rat brown adipose tissue and extract of E. coli expressing UCP3).

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